Aquatic Toxicity

STILLMEADOW, Inc. conducts a variety of aquatic toxicity tests for both freshwater and saltwater project requirements. We provide GLP compliant environmental services designed to meet the regulations of government and other agencies under EPA, OCSPP and OECD guidelines. STILLMEADOW, Inc. maintains an extensive culture facility focused on managing regimented productions of test organisms commonly used. Tests are designed based on guidelines and client specific requirements and performed in environmentally controlled chambers.

GLP Acute and Chronic Aquatic Tests

Freshwater and saltwater organisms are exposed to a series of five test concentrations in a static and static-renewal test system in order to gather toxicity data for assessment of potential environmental hazards of the product to aquatic life. The following tests are the most common requested and can be customized to meet a client’s specific needs:

  • Daphnia spp. Static 48-hr Acute Toxicity Test (OCSPP 850.1010, OECD 202)
  • Daphnia spp. Static 21-day Chronic Toxicity Test (OCSPP 850.1300, OECD 211)
  • Static 96-hr. Fish Acute Toxicity Test (OCSPP 850.1075, OECD 203)
  • Mysidopsis bahia Static 96-hr Acute Toxicity Test (OCSPP 850.1035)
  • Mysidopsis bahia Static 28-day Chronic Toxicity Test (OCSPP 850.1350)
  • Early life stage static toxicity test (OCSPP 850.1400 or OECD 210)
  • Freshwater fish testing, Tier 1, microbial pest control agent (MPCA) test (OCSPP 885.4200)
  • Freshwater aquatic invertebrate testing, Tier 1, microbial control agent (MPCA)  test (OCSPP 885.4240)

Microbial Pest Control Agent (MPCA) Tests

Freshwater or saltwater organisms are administered the active MPCA test substance through environmental and dietary (fish only) exposure. Additionally, sterile filtrate or heat-killed test substance control groups are used to quantify any potential toxicity un-related to the active substance. Typically, only a single limit dose is used, however, multiple concentrations can be utilized per sponsor’s unique requirements.


The above guideline-driven tests provide a general toxicological response picture and additional add-ons such as dose level verifications, histology, necropsy, additional water quality parameters, etc. can be used to complete a full assessment of the toxicity potential of a product.
Contact us to discuss an aquatic toxicity test design that meets your needs.




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